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Image Search Results
Journal: bioRxiv
Article Title: Cortical distribution of neurofilaments associates with pathological hallmarks and MRI measures of atrophy and diffusivity in Parkinson’s disease
doi: 10.1101/2022.08.10.503440
Figure Lengend Snippet: NfL accumulation and fragmentation, and loss of neurofilament colocalization in PD and PDD/DLB. Maximum projections (z-stacks n=6 μm) of fluorescence stainings of NfL (red) and p-NfM/H (green) together with DAPI (blue) in the parahippocampal gyrus of a representative control ( left , control number 10 in Supplementary Table 1, online resource 1), PD ( middle , PD number 3 in Supplementary Table 1, online resource 1) and PDD/DLB ( right , here a DLB donor, PDD/DLB number 12 in Supplementary Table 1, online resource 1) are shown. a , b , and c show the difference in NfL staining pattern in control, PD and PDD/DLB, respectively. NfL was markedly accumulated in neuronal somas and proximal axons in PDD/DLB cases, reflecting the higher NfL immunoreactivity described quantitatively in . High NfL immunoreactivity was found in neuronal somas showing neurodegenerative morphological features, such as c neurons with nuclear fragmentation and swelling (*), and d corkscrew (**) and ballooned (***) appearances (panel d shows a zoom-in 3D surface-reconstruction of neurodegenerative neurons). Moreover, c NfL accumulation was also identified in a proximal axon showing fragmentation (arrow), closely tighten by a glial cell (#); e shows the zoom-in 3D surface-reconstruction of the white square in c , showing axonal fragmented morphology (arrow) and a glial cell wrapping the degenerating axon (#). f , g and h show the colocalization (yellow) of NfL and p-NfM/H in control, PD and PDD/DLB, respectively, which was reduced in PD and PDD/DLB. Moreover, accumulation and fragmentation of NfL staining patterns were observed in several axons, where g NfL seemed to fragment within a p-NfM/H axon in PD (dashed arrow) and h axonal swellings intermitted axonal fragmentations in PDD/DLB (arrows). i shows the zoom-in 3D surface-reconstruction of the white square in h , clearly showing fragmentation of the NfL positive axon (arrows). Legend: DLB: Dementia with Lewy Bodies; NfL: neurofilament light chain; PD: Parkinson’s disease; PDD: Parkinson’s disease dementia; p-NfM/H: phosphorylated neurofilament medium and heavy chain
Article Snippet: After scanning, the images were deconvoluted using CMLE algorithms in Huygens Professional (Scientific Volume imaging; Huygens, The Netherlands; https://svi.nl/Huygens-Professional ), and their maximum projections (ImageJ Fiji, National Institute of Health USA; https://imagej.nih.gov/ij/ ) or
Techniques: Fluorescence, Control, Staining